Hotspot

rpl32-trnL-CUA

SSC · 113597-114385 · 789 bp

Back to Cymatocarpus

Pi 0.0364
MarkerSeek score 79.20
Primer available yes
Label rpl32-trnL-CUA

Feature Metadata

label_name
rpl32-trnL-CUA
feature_type
igs
region
SSC
length_bp
789
variable_sites
43
pi
0.0364
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-CUA_p1 GGAAGGGATATTGGACATCGT TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.2 57.9 778-926 bp 1.0 72.8
rpl32-trnL-CUA_p2 GGAAGGGATATTGGACATCGT AGCAGCGTGTCTACCAATTT 47.6 45.0 57.2 57.8 771-919 bp 1.0 72.3
rpl32-trnL-CUA_p3 GGAAGGGATATTGGACATCGT CCTAAGAGCAGCGTGTCTAC 47.6 55.0 57.2 57.8 777-925 bp 1.0 72.2
rpl32-trnL-CUA_p4 GGAAGGGATATTGGACATCGT CTAAGAGCAGCGTGTCTACC 47.6 55.0 57.2 57.8 776-924 bp 1.0 72.2
rpl32-trnL-CUA_p5 GGAAGGGATATTGGACATCGT GAGCAGCGTGTCTACCAATT 47.6 50.0 57.2 58.3 772-920 bp 1.0 71.6