Hotspot

petD-rpoA

LSC · 77587-77779 · 193 bp

Back to Cymatocarpus

Pi 0.0415

MarkerSeek score 64.30

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 193
variable_sites: 12
pi: 0.0415
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GCCCTGTGGTTAGGTATTGG	GAAGCCTCCCGGAATTTGAT	55.0	50.0	57.9	57.9	595-599 bp	1.0	81.1
petD-rpoA_p2	GGCCCTGTGGTTAGGTATTG	GAAGCCTCCCGGAATTTGAT	55.0	50.0	57.9	57.9	596-600 bp	1.0	81.1
petD-rpoA_p3	CCGTCTTTTTGATTGGCACC	GAAGCCTCCCGGAATTTGAT	50.0	50.0	57.9	57.9	621-625 bp	1.0	80.9
petD-rpoA_p4	TACCATTGCCTGTAACGTGG	GAAGCCTCCCGGAATTTGAT	50.0	50.0	57.9	57.9	875-879 bp	1.0	80.3
petD-rpoA_p5	CTCGAGCCGGATGATGAAAA	GAAGCCTCCCGGAATTTGAT	50.0	50.0	58.1	57.9	1080-1084 bp	0.666667	67.1