Hotspot

trnL-UUA-trnF-UUC

LSC · 47226-48169 · 944 bp

Back to Cymatocarpus

Pi 0.0954

MarkerSeek score 91.70

Primer available yes

Label trnL-UUA-trnF-UUC

Feature Metadata

label_name: trnL-UUA-trnF-UUC
feature_type: igs
region: LSC
length_bp: 944
variable_sites: 125
pi: 0.0954
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 91.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UUA-trnF-UUC_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	973-1034 bp	1.0	87.8
trnL-UUA-trnF-UUC_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	970-1031 bp	1.0	84.5
trnL-UUA-trnF-UUC_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	962-1023 bp	1.0	83.9
trnL-UUA-trnF-UUC_p4	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAA	50.0	50.0	57.5	57.4	949-1010 bp	1.0	83.4
trnL-UUA-trnF-UUC_p5	AATCGTGAGGGTTCAAGTCC	TTCAGTCCTCTGCTCTACCA	50.0	50.0	57.5	57.4	950-1011 bp	1.0	83.4