Hotspot

rps14-psaB

LSC · 36965-37093 · 129 bp

Back to Cymatocarpus

Pi 0.0207

MarkerSeek score 64.20

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 129
variable_sites: 4
pi: 0.0207
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	CACTTAGCGACGGAATCTGA	TCTTGATTGCCTCCACATCG	50.0	50.0	57.4	58.0	279-280 bp	1.0	76.4
rps14-psaB_p2	TCACTTAGCGACGGAATCTG	TCTTGATTGCCTCCACATCG	50.0	50.0	57.4	58.0	280-281 bp	1.0	76.4
rps14-psaB_p3	ACTTAGCGACGGAATCTGAC	TCTTGATTGCCTCCACATCG	50.0	50.0	57.4	58.0	278-279 bp	1.0	76.4
rps14-psaB_p4	CACTTAGCGACGGAATCTGA	ACATCGGGCAAATTCGGTTA	50.0	45.0	57.4	57.9	265-266 bp	1.0	75.8
rps14-psaB_p5	TCACTTAGCGACGGAATCTG	ACATCGGGCAAATTCGGTTA	50.0	45.0	57.4	57.9	266-267 bp	1.0	75.8