Hotspot

psbC-trnS-UCA

LSC · 34910-35091 · 182 bp

Back to Cymatocarpus

Pi 0.0258
MarkerSeek score 64.80
Primer available yes
Label psbC-trnS-UCA

Feature Metadata

label_name
psbC-trnS-UCA
feature_type
igs
region
LSC
length_bp
182
variable_sites
7
pi
0.0258
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UCA_p1 TCGTGGGTCATTTATGGCAC GATGGCTCCGGTCTTGAAAA 50.0 50.0 58.3 58.2 363-364 bp 1.0 78.5
psbC-trnS-UCA_p2 TCGTGGGTCATTTATGGCAC GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.3 58.2 314-315 bp 1.0 78.5
psbC-trnS-UCA_p3 CGGCAGCAGGATTTGAAAAA GGGTTCGAATCCCTCTCTCT 45.0 55.0 57.6 58.2 275-276 bp 1.0 77.4
psbC-trnS-UCA_p4 CGGCAGCAGGATTTGAAAAA GATGGCTCCGGTCTTGAAAA 45.0 50.0 57.6 58.2 324-325 bp 1.0 77.3
psbC-trnS-UCA_p5 TTCGTGGGTCATTTATGGCA GATGGCTCCGGTCTTGAAAA 45.0 50.0 57.5 58.2 364-365 bp 1.0 76.6