Hotspot

trnR-AGA-atpA

LSC · 9305-9613 · 309 bp

Back to Cymatocarpus

Pi 0.0261

MarkerSeek score 62.20

Primer available yes

Label trnR-AGA-atpA

Feature Metadata

label_name: trnR-AGA-atpA
feature_type: igs
region: LSC
length_bp: 309
variable_sites: 12
pi: 0.0261
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-AGA-atpA_p1	GGTTCAAATCCTATTGGACGC	ACATTAACCGCTGAAGCAGA	47.6	45.0	57.3	57.5	410-415 bp	1.0	66.2
trnR-AGA-atpA_p2	GGTTCAAATCCTATTGGACGC	AGACATTAACCGCTGAAGCA	47.6	45.0	57.3	57.5	412-417 bp	1.0	66.1
trnR-AGA-atpA_p3	CGAAAAGCGTCCATTGTCTA	ACATTAACCGCTGAAGCAGA	45.0	45.0	56.2	57.5	466-471 bp	1.0	64.8
trnR-AGA-atpA_p4	CGAAAAGCGTCCATTGTCTA	AGACATTAACCGCTGAAGCA	45.0	45.0	56.2	57.5	468-473 bp	1.0	64.7
trnR-AGA-atpA_p5	GGTTCAAATCCTATTGGACGC	GACATTAACCGCTGAAGCAG	47.6	50.0	57.3	57.2	411-416 bp	1.0	63.4