Hotspot

trnS-AGC-trnG-GGA

LSC · 7618-8277 · 660 bp

Back to Cymatocarpus

Pi 0.0291

MarkerSeek score 73.30

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 26
pi: 0.0291
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	CCAACGCTTTAGTCCACTCA	TGAATCAAACCGAGAGACCC	50.0	50.0	57.8	57.2	757-801 bp	1.0	77.9
trnS-AGC-trnG-GGA_p2	AACGCTTTAGTCCACTCAGC	TGAATCAAACCGAGAGACCC	50.0	50.0	58.2	57.2	755-799 bp	1.0	77.7
trnS-AGC-trnG-GGA_p3	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAGAGACCC	50.0	50.0	57.6	57.2	752-796 bp	1.0	76.0
trnS-AGC-trnG-GGA_p4	TCCAACGCTTTAGTCCACTC	TGAATCAAACCGAGAGACCC	50.0	50.0	57.5	57.2	758-802 bp	1.0	75.6
trnS-AGC-trnG-GGA_p5	CCAACGCTTTAGTCCACTCA	GAATCAAACCGAGAGACCCT	50.0	50.0	57.8	56.9	756-800 bp	1.0	75.6