Hotspot

rps3

Genome · 94205-94915 · 711 bp

Back to Cyclotella

Pi 0.123

MarkerSeek score 82.00

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: Genome
length_bp: 711
variable_sites: 165
pi: 0.123
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TTGCCGATGAAGGTCCAAAA	AAATTAAAAGCCGCACGAGC	45.0	45.0	58.0	58.0	1179-1328 bp	0.5	71.7
rps3_p2	TTTGCCGATGAAGGTCCAAA	AAATTAAAAGCCGCACGAGC	45.0	45.0	58.0	58.0	1180-1329 bp	0.5	71.7
rps3_p3	TTTTGCCGATGAAGGTCCAA	AAATTAAAAGCCGCACGAGC	45.0	45.0	58.0	58.0	1181-1330 bp	0.5	71.7
rps3_p4	TTTTTGCCGATGAAGGTCCA	AAATTAAAAGCCGCACGAGC	45.0	45.0	58.0	58.0	1182-1331 bp	0.5	71.7
rps3_p5	AGGTTTTTGCCGATGAAGGT	AAATTAAAAGCCGCACGAGC	45.0	45.0	58.0	58.0	1334 bp	0.166667	46.4