Hotspot

atpD

Genome · 11657-12223 · 567 bp

Back to Cyclotella

Pi 0.206

MarkerSeek score 82.30

Primer available yes

Label atpD

Feature Metadata

label_name: atpD
feature_type: gene
region: Genome
length_bp: 567
variable_sites: 220
pi: 0.206
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpD_p1	AAGCCTTCACCTACAGGGAT	AATAGAGCAATCGCGACGTT	50.0	45.0	58.0	58.1	1105 bp	0.166667	45.8
atpD_p2	AAGCCTTCACCTACAGGGAT	ATAGAGCAATCGCGACGTTT	50.0	45.0	58.0	58.1	1104 bp	0.166667	45.8
atpD_p3	AGCCTTCACCTACAGGGATT	AATAGAGCAATCGCGACGTT	50.0	45.0	58.0	58.1	1104 bp	0.166667	45.8
atpD_p4	AGCCTTCACCTACAGGGATT	ATAGAGCAATCGCGACGTTT	50.0	45.0	58.0	58.1	1103 bp	0.166667	45.8
atpD_p5	CATCAAGCCCATAAACCCGA	ATAGAGCAATCGCGACGTTT	50.0	45.0	57.9	58.1	932 bp	0.166667	45.6