Hotspot

atpA

Genome · 10096-11610 · 1515 bp

Back to Cyclotella

Pi 0.128
MarkerSeek score 80.30
Primer available yes
Label atpA

Feature Metadata

label_name
atpA
feature_type
gene
region
Genome
length_bp
1515
variable_sites
378
pi
0.128
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA_p1 ATACTCCAACCACCCCAAGA TGCACGGGCTTTATACGATT 50.0 45.0 57.9 57.7 2521-2538 bp 0.333333 60.9
atpA_p2 CTCCAACCACCCCAAGAATC TGCACGGGCTTTATACGATT 55.0 45.0 58.2 57.7 2518-2535 bp 0.333333 60.0
atpA_p3 TACTCCAACCACCCCAAGAA TGCACGGGCTTTATACGATT 50.0 45.0 58.2 57.7 2520-2537 bp 0.333333 59.9
atpA_p4 ATACCTTGACGCCACATTGG TGCACGGGCTTTATACGATT 50.0 45.0 58.2 57.7 2461-2478 bp 0.333333 59.4
atpA_p5 AACCCAATGCCAAATTGCTG TGCACGGGCTTTATACGATT 45.0 45.0 57.8 57.7 2647 bp 0.166667 42.6