Hotspot

psaC-psbA

Genome · 117072-117872 · 801 bp

Back to Cyclotella

Pi 0.346

MarkerSeek score 79.30

Primer available yes

Label psaC-psbA

Feature Metadata

label_name: psaC-psbA
feature_type: igs
region: Genome
length_bp: 801
variable_sites: 474
pi: 0.346
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-psbA_p1	TGAGCCTGATACACGAAACG	TCGTAGCTGCACATGGTTAC	50.0	50.0	58.0	58.0	1857 bp	0.166667	46.6
psaC-psbA_p2	TGAGCCTGATACACGAAACG	CGTAGCTGCACATGGTTACT	50.0	50.0	58.0	58.0	1856 bp	0.166667	46.5
psaC-psbA_p3	TGAGCCTGATACACGAAACG	TGCATCTTACATGGGTCGTG	50.0	50.0	58.0	58.0	2231 bp	0.166667	46.4
psaC-psbA_p4	TTGGGATGGATGTAAAGCCG	TCGTAGCTGCACATGGTTAC	50.0	50.0	57.9	58.0	1299 bp	0.166667	46.2
psaC-psbA_p5	TTGGGATGGATGTAAAGCCG	CGTAGCTGCACATGGTTACT	50.0	50.0	57.9	58.0	1298 bp	0.166667	46.2