Hotspot

rpl13-rps9

Genome · 103683-104045 · 363 bp

Back to Cyclotella

Pi 0.445

MarkerSeek score 68.00

Primer available yes

Label rpl13-rps9

Feature Metadata

label_name: rpl13-rps9
feature_type: igs
region: Genome
length_bp: 363
variable_sites: 221
pi: 0.445
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.53
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl13-rps9_p1	CCTGGCCATCCTGGTAAATC	TTCGTGCTTTCCGTAAACCA	55.0	45.0	58.0	58.0	845-991 bp	0.333333	64.8
rpl13-rps9_p2	GCATCGGACCCACTATTGTT	TTCGTGCTTTCCGTAAACCA	50.0	45.0	58.0	58.0	991-1137 bp	0.333333	64.6
rpl13-rps9_p3	TGGTCGTAAATCGGCAGATG	TTCGTGCTTTCCGTAAACCA	50.0	45.0	58.1	58.0	1169-1315 bp	0.333333	64.4
rpl13-rps9_p4	CCTGGCCATCCTGGTAAATC	CTTCCCATTTCCCGGAACAA	55.0	50.0	58.0	58.1	702 bp	0.166667	45.9
rpl13-rps9_p5	CCTGGCCATCCTGGTAAATC	TTGGCCAGTTAATCCACCAC	55.0	50.0	58.0	58.1	849 bp	0.166667	45.9