Hotspot

psbA-psaC

Genome · 80681-81481 · 801 bp

Back to Cyclotella

Pi 0.334

MarkerSeek score 80.00

Primer available yes

Label psbA-psaC

Feature Metadata

label_name: psbA-psaC
feature_type: igs
region: Genome
length_bp: 801
variable_sites: 460
pi: 0.334
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-psaC_p1	TCGTAGCTGCACATGGTTAC	TGAGCCTGATACACGAAACG	50.0	50.0	58.0	58.0	1857 bp	0.166667	46.6
psbA-psaC_p2	CGTAGCTGCACATGGTTACT	TGAGCCTGATACACGAAACG	50.0	50.0	58.0	58.0	1856 bp	0.166667	46.5
psbA-psaC_p3	TGCATCTTACATGGGTCGTG	TGAGCCTGATACACGAAACG	50.0	50.0	58.0	58.0	2231 bp	0.166667	46.4
psbA-psaC_p4	TCGTAGCTGCACATGGTTAC	TTGGGATGGATGTAAAGCCG	50.0	50.0	58.0	57.9	1299 bp	0.166667	46.2
psbA-psaC_p5	CGTAGCTGCACATGGTTACT	TTGGGATGGATGTAAAGCCG	50.0	50.0	58.0	57.9	1298 bp	0.166667	46.2