Hotspot

trnK-UUU-ycf12

Genome · 53769-53940 · 172 bp

Back to Cyclotella

Pi 0.117

MarkerSeek score 82.70

Primer available yes

Label trnK-UUU-ycf12

Feature Metadata

label_name: trnK-UUU-ycf12
feature_type: igs
region: Genome
length_bp: 172
variable_sites: 43
pi: 0.117
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-ycf12_p1	TCGAACCCAGAACAAATCGG	AGCTGACCTATAACTTGCCA	50.0	45.0	58.2	56.2	301-331 bp	1.0	76.3
trnK-UUU-ycf12_p2	GGACTCGAACCCAGAACAAA	AGCTGACCTATAACTTGCCA	50.0	45.0	57.8	56.2	305-335 bp	1.0	75.8
trnK-UUU-ycf12_p3	TCGAACCCAGAACAAATCGG	AGCTGACCTATAACTTGCCAA	50.0	42.9	58.2	57.0	301-331 bp	1.0	74.4
trnK-UUU-ycf12_p4	GGACTCGAACCCAGAACAAA	AGCTGACCTATAACTTGCCAA	50.0	42.9	57.8	57.0	305-335 bp	1.0	74.0
trnK-UUU-ycf12_p5	CGAACCCAGAACAAATCGGT	AGCTGACCTATAACTTGCCA	50.0	45.0	58.5	56.2	300-330 bp	1.0	74.0