primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	atpA	1	ATACTCCAACCACCCCAAGA	TGCACGGGCTTTATACGATT	20	20	50.0	45.0	57.9	57.7	0.0	12.7	0.372883	10096	11610	2521-2538	0.333333	60.9
atpA_p2	atpA	2	CTCCAACCACCCCAAGAATC	TGCACGGGCTTTATACGATT	20	20	55.0	45.0	58.2	57.7	0.0	12.7	0.479713	10096	11610	2518-2535	0.333333	60.0
atpA_p3	atpA	3	TACTCCAACCACCCCAAGAA	TGCACGGGCTTTATACGATT	20	20	50.0	45.0	58.2	57.7	0.0	12.7	0.496711	10096	11610	2520-2537	0.333333	59.9
atpA_p4	atpA	4	ATACCTTGACGCCACATTGG	TGCACGGGCTTTATACGATT	20	20	50.0	45.0	58.2	57.7	0.0	12.7	0.568943	10096	11610	2461-2478	0.333333	59.4
atpA_p5	atpA	5	AACCCAATGCCAAATTGCTG	TGCACGGGCTTTATACGATT	20	20	45.0	45.0	57.8	57.7	0.0	12.7	0.507198	10096	11610	2647-2647	0.166667	42.6
atpD_p1	atpD	1	AAGCCTTCACCTACAGGGAT	AATAGAGCAATCGCGACGTT	20	20	50.0	45.0	58.0	58.1	0.0	12.9	0.104842	11657	12223	1105-1105	0.166667	45.8
atpD_p2	atpD	2	AAGCCTTCACCTACAGGGAT	ATAGAGCAATCGCGACGTTT	20	20	50.0	45.0	58.0	58.1	0.0	12.9	0.104842	11657	12223	1104-1104	0.166667	45.8
atpD_p3	atpD	3	AGCCTTCACCTACAGGGATT	AATAGAGCAATCGCGACGTT	20	20	50.0	45.0	58.0	58.1	0.0	12.9	0.104842	11657	12223	1104-1104	0.166667	45.8
atpD_p4	atpD	4	AGCCTTCACCTACAGGGATT	ATAGAGCAATCGCGACGTTT	20	20	50.0	45.0	58.0	58.1	0.0	12.9	0.104842	11657	12223	1103-1103	0.166667	45.8
atpD_p5	atpD	5	CATCAAGCCCATAAACCCGA	ATAGAGCAATCGCGACGTTT	20	20	50.0	45.0	57.9	58.1	0.0	12.9	0.128764	11657	12223	932-932	0.166667	45.6
trnK-UUU-ycf12_p1	trnK-UUU-ycf12	1	TCGAACCCAGAACAAATCGG	AGCTGACCTATAACTTGCCA	20	20	50.0	45.0	58.2	56.2	0.0	0.0	1.967640	53769	53940	301-331	1.000000	76.3
trnK-UUU-ycf12_p2	trnK-UUU-ycf12	2	GGACTCGAACCCAGAACAAA	AGCTGACCTATAACTTGCCA	20	20	50.0	45.0	57.8	56.2	0.0	0.0	2.020818	53769	53940	305-335	1.000000	75.8
trnK-UUU-ycf12_p3	trnK-UUU-ycf12	3	TCGAACCCAGAACAAATCGG	AGCTGACCTATAACTTGCCAA	20	21	50.0	42.9	58.2	57.0	0.0	0.0	2.197574	53769	53940	301-331	1.000000	74.4
trnK-UUU-ycf12_p4	trnK-UUU-ycf12	4	GGACTCGAACCCAGAACAAA	AGCTGACCTATAACTTGCCAA	20	21	50.0	42.9	57.8	57.0	0.0	0.0	2.250751	53769	53940	305-335	1.000000	74.0
trnK-UUU-ycf12_p5	trnK-UUU-ycf12	5	CGAACCCAGAACAAATCGGT	AGCTGACCTATAACTTGCCA	20	20	50.0	45.0	58.5	56.2	0.0	0.0	2.251566	53769	53940	300-330	1.000000	74.0
ycf90_p1	ycf90	1	TGTTGTATTGGGCTTGCTGT	ACGCAAGATCGTCAACAAGT	20	20	45.0	45.0	58.0	58.1	0.0	0.0	0.140179	57498	58868	2560-2576	0.333333	56.6
ycf90_p2	ycf90	2	TGTTGTATTGGGCTTGCTGT	GTTTTTGGAACACCACGTCC	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.203836	57498	58868	2593-2609	0.333333	56.1
ycf90_p3	ycf90	3	TGTTGTATTGGGCTTGCTGT	TTTTTGGAACACCACGTCCT	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.137820	57498	58868	2608-2608	0.166667	45.6
ycf90_p4	ycf90	4	AACTTGCCAAAGGTATGGGG	TTTTTGGAACACCACGTCCT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.194585	57498	58868	2706-2706	0.166667	45.1
ycf90_p5	ycf90	5	AACTTGCCAAAGGTATGGGG	ACGCAAGATCGTCAACAAGT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.196944	57498	58868	2674-2674	0.166667	45.1
psbA-psaC_p1	psbA-psaC	1	TCGTAGCTGCACATGGTTAC	TGAGCCTGATACACGAAACG	20	20	50.0	50.0	58.0	58.0	1.0	0.0	0.013783	80681	81481	1857-1857	0.166667	46.6
psbA-psaC_p2	psbA-psaC	2	CGTAGCTGCACATGGTTACT	TGAGCCTGATACACGAAACG	20	20	50.0	50.0	58.0	58.0	1.0	0.0	0.019346	80681	81481	1856-1856	0.166667	46.5
psbA-psaC_p3	psbA-psaC	3	TGCATCTTACATGGGTCGTG	TGAGCCTGATACACGAAACG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.031965	80681	81481	2231-2231	0.166667	46.4
psbA-psaC_p4	psbA-psaC	4	TCGTAGCTGCACATGGTTAC	TTGGGATGGATGTAAAGCCG	20	20	50.0	50.0	58.0	57.9	1.0	0.0	0.055917	80681	81481	1299-1299	0.166667	46.2
psbA-psaC_p5	psbA-psaC	5	CGTAGCTGCACATGGTTACT	TTGGGATGGATGTAAAGCCG	20	20	50.0	50.0	58.0	57.9	1.0	0.0	0.061479	80681	81481	1298-1298	0.166667	46.2
ccsA_p1	ccsA	1	CGAGTTGTTTCAGCCCCTAA	TCTGCTGTTCGTCGAGAAAC	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.411623	81818	82873	1541-1583	0.333333	56.9
ccsA_p2	ccsA	2	CGGCTTTACATCCATCCCAA	CTGCTGTTCGTCGAGAAACT	20	20	50.0	50.0	57.9	58.2	0.0	10.3	0.279152	81818	82873	1467-1467	0.166667	44.4
ccsA_p3	ccsA	3	CGGCTTTACATCCATCCCAA	GTCTGCTGTTCGTCGAGAAA	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.284046	81818	82873	1469-1469	0.166667	44.4
ccsA_p4	ccsA	4	CGGCTTTACATCCATCCCAA	TCTGCTGTTCGTCGAGAAAC	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.284046	81818	82873	1468-1468	0.166667	44.4
ccsA_p5	ccsA	5	CGAGTTGTTTCAGCCCCTAA	CTGCTGTTCGTCGAGAAACT	20	20	50.0	50.0	57.8	58.2	0.0	10.3	0.406729	81818	82873	1582-1582	0.166667	43.4
thiS-trnQ-UUG_p1	thiS-trnQ-UUG	1	TTTGGGTCAATCCAGGCAAA	CTTTGACTCCGCCATTCGTA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.098683	85284	86146	1423-1423	0.166667	45.9
thiS-trnQ-UUG_p2	thiS-trnQ-UUG	2	TTTGGGTCAATCCAGGCAAA	TTTGACTCCGCCATTCGTAG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.098683	85284	86146	1422-1422	0.166667	45.9
thiS-trnQ-UUG_p3	thiS-trnQ-UUG	3	TTTTGGGTCAATCCAGGCAA	CTTTGACTCCGCCATTCGTA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.098683	85284	86146	1424-1424	0.166667	45.9
thiS-trnQ-UUG_p4	thiS-trnQ-UUG	4	TTTTGGGTCAATCCAGGCAA	TTTGACTCCGCCATTCGTAG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.098683	85284	86146	1423-1423	0.166667	45.9
thiS-trnQ-UUG_p5	thiS-trnQ-UUG	5	TTTTTGGGTCAATCCAGGCA	TTTGACTCCGCCATTCGTAG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.098683	85284	86146	1424-1424	0.166667	45.9
dnaK_p1	dnaK	1	CCCGCAAAAGTAACACGTTC	TGAGTCATAGGTCCTCTTGCA	20	21	50.0	47.6	58.0	58.2	0.0	0.0	1.206737	88284	90071	2778-2826	0.333333	51.2
dnaK_p2	dnaK	2	TTCTCGGGCTATTCTTGCAC	TGAGTCATAGGTCCTCTTGCA	20	21	50.0	47.6	58.0	58.2	0.0	0.0	1.203998	88284	90071	2935-2983	0.333333	51.1
dnaK_p3	dnaK	3	ATCCCGCAAAAGTAACACGT	TGAGTCATAGGTCCTCTTGCA	20	21	45.0	47.6	58.1	58.2	0.0	0.0	1.292936	88284	90071	2780-2828	0.333333	50.5
dnaK_p4	dnaK	4	TGCTGGAATTAATGGTCCCG	TGAGTCATAGGTCCTCTTGCA	20	21	50.0	47.6	57.9	58.2	4.1	0.0	1.231156	88284	90071	2941-2941	0.166667	36.8
dnaK_p5	dnaK	5	GCTGGAATTAATGGTCCCGT	TGAGTCATAGGTCCTCTTGCA	20	21	50.0	47.6	57.9	58.2	4.1	0.0	1.232604	88284	90071	2940-2940	0.166667	36.8
rpl3_p1	rpl3	1	GGGTTGTTCGAACACCATCT	AACGAAACGTCACTCCACAA	20	20	50.0	45.0	58.1	58.0	32.1	0.0	0.120727	90340	91020	1078-1078	0.166667	45.7
rpl3_p2	rpl3	2	GGGTTGTTCGAACACCATCT	AGCACGACCTGTTCCTTTTT	20	20	50.0	45.0	58.1	57.9	32.1	0.0	0.160573	90340	91020	1490-1490	0.166667	45.4
rpl3_p3	rpl3	3	GGGTTGTTCGAACACCATCT	TTTGTTTTTGGCAGCACGTT	20	20	50.0	40.0	58.1	57.9	32.1	0.0	0.193845	90340	91020	1693-1693	0.166667	45.1
rpl3_p4	rpl3	4	GGGTTGTTCGAACACCATCT	TTTTGTTTTTGGCAGCACGT	20	20	50.0	40.0	58.1	57.9	32.1	0.0	0.193845	90340	91020	1694-1694	0.166667	45.1
rpl3_p5	rpl3	5	GGGTTGTTCGAACACCATCT	TAAACCTCCGCACATGTCAG	20	20	50.0	50.0	58.1	57.9	32.1	0.0	0.201097	90340	91020	1667-1667	0.166667	45.1
rpl4_p1	rpl4	1	TTGTGGAGTGACGTTTCGTT	TCTGAGAATGCTGAAAGGGC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.132738	91234	91887	1266-1266	0.166667	45.6
rpl4_p2	rpl4	2	TTGTGGAGTGACGTTTCGTT	TGAGAATGCTGAAAGGGCTC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.132738	91234	91887	1264-1264	0.166667	45.6
rpl4_p3	rpl4	3	TTGTGGAGTGACGTTTCGTT	CTGAGAATGCTGAAAGGGCT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.138710	91234	91887	1265-1265	0.166667	45.6
rpl4_p4	rpl4	4	TTGTGGAGTGACGTTTCGTT	CTCCGCCACGATGTCTAATC	20	20	45.0	55.0	58.0	58.2	0.0	0.0	0.240754	91234	91887	1367-1367	0.166667	44.7
rpl4_p5	rpl4	5	GAGCTCCTGGTTCAATTGGT	TGAGAATGCTGAAAGGGCTC	20	20	50.0	50.0	57.8	57.9	0.9	0.0	0.326377	91234	91887	1476-1476	0.166667	44.1
rps3_p1	rps3	1	TTGCCGATGAAGGTCCAAAA	AAATTAAAAGCCGCACGAGC	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.034518	94205	94915	1179-1328	0.500000	71.7
rps3_p2	rps3	2	TTTGCCGATGAAGGTCCAAA	AAATTAAAAGCCGCACGAGC	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.034518	94205	94915	1180-1329	0.500000	71.7
rps3_p3	rps3	3	TTTTGCCGATGAAGGTCCAA	AAATTAAAAGCCGCACGAGC	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.034518	94205	94915	1181-1330	0.500000	71.7
rps3_p4	rps3	4	TTTTTGCCGATGAAGGTCCA	AAATTAAAAGCCGCACGAGC	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.034518	94205	94915	1182-1331	0.500000	71.7
rps3_p5	rps3	5	AGGTTTTTGCCGATGAAGGT	AAATTAAAAGCCGCACGAGC	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.027435	94205	94915	1334-1334	0.166667	46.4
rpl5_p1	rpl5	1	GTGTTTTTGGTCCTGTTGCC	ACACGCAAACCAGGTTTACT	20	20	50.0	45.0	58.1	58.0	0.0	17.6	0.112413	96752	97474	1333-1372	0.333333	58.6
rpl5_p2	rpl5	2	GTGTTTTTGGTCCTGTTGCC	GACTGATTGCCCTCTCCATG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.100859	96752	97474	1688-1688	0.166667	45.9
rpl5_p3	rpl5	3	GTGTTTTTGGTCCTGTTGCC	GCCTGCCTACCTGTCATTAC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.110484	96752	97474	1465-1465	0.166667	45.8
rpl5_p4	rpl5	4	GTGTTTTTGGTCCTGTTGCC	TTCCATTAGACATCGCCTGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.111852	96752	97474	1479-1479	0.166667	45.8
rpl5_p5	rpl5	5	GTGTTTTTGGTCCTGTTGCC	GATCGACTGATTGCCCTCTC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.212847	96752	97474	1692-1692	0.166667	45.0
rpl6_p1	rpl6	1	GTAATGACAGGTAGGCAGGC	TTATAGGACTCCCCGCGAAT	20	20	55.0	50.0	58.0	58.0	0.0	8.2	0.050443	97926	98525	1180-1180	0.166667	46.3
rpl6_p2	rpl6	2	GCAGGCGATGTCTAATGGAA	TTATAGGACTCCCCGCGAAT	20	20	50.0	50.0	58.0	58.0	0.0	8.2	0.051811	97926	98525	1166-1166	0.166667	46.3
rpl6_p3	rpl6	3	AGTAAACCTGGTTTGCGTGT	TTATAGGACTCCCCGCGAAT	20	20	45.0	50.0	58.0	58.0	30.1	8.2	0.052372	97926	98525	1273-1273	0.166667	46.2
rpl6_p4	rpl6	4	GTAATGACAGGTAGGCAGGC	TAAGCGTGGTCTTTCGACTG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.105399	97926	98525	843-843	0.166667	45.8
rpl6_p5	rpl6	5	GCAGGCGATGTCTAATGGAA	TAAGCGTGGTCTTTCGACTG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.106767	97926	98525	829-829	0.166667	45.8
rpl13_p1	rpl13	1	TGGTCGTAAATCGGCAGATG	TTCGTGCTTTCCGTAAACCA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.114583	103194	103682	1169-1315	0.333333	64.4
rpl13_p2	rpl13	2	TGGTCGTAAATCGGCAGATG	CTTCCCATTTCCCGGAACAA	20	20	50.0	50.0	58.1	58.1	0.0	0.7	0.147539	103194	103682	1026-1026	0.166667	45.5
rpl13_p3	rpl13	3	TGGTCGTAAATCGGCAGATG	TTGGCCAGTTAATCCACCAC	20	20	50.0	50.0	58.1	58.1	0.0	5.1	0.147539	103194	103682	1173-1173	0.166667	45.5
rpl13_p4	rpl13	4	ACTCGACAATTTGGACGCTT	TTCGTGCTTTCCGTAAACCA	20	20	45.0	45.0	58.1	58.0	11.4	0.0	0.170219	103194	103682	1908-1908	0.166667	45.3
rpl13_p5	rpl13	5	TGGTCGTAAATCGGCAGATG	TCTGCTTGGCCAGTTAATCC	20	20	50.0	50.0	58.1	57.9	0.0	5.1	0.194182	103194	103682	1178-1178	0.166667	45.1
rpl13-rps9_p1	rpl13-rps9	1	CCTGGCCATCCTGGTAAATC	TTCGTGCTTTCCGTAAACCA	20	20	55.0	45.0	58.0	58.0	5.5	0.0	0.062289	103683	104045	845-991	0.333333	64.8
rpl13-rps9_p2	rpl13-rps9	2	GCATCGGACCCACTATTGTT	TTCGTGCTTTCCGTAAACCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.087559	103683	104045	991-1137	0.333333	64.6
rpl13-rps9_p3	rpl13-rps9	3	TGGTCGTAAATCGGCAGATG	TTCGTGCTTTCCGTAAACCA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.114583	103683	104045	1169-1315	0.333333	64.4
rpl13-rps9_p4	rpl13-rps9	4	CCTGGCCATCCTGGTAAATC	CTTCCCATTTCCCGGAACAA	20	20	55.0	50.0	58.0	58.1	5.5	0.7	0.095246	103683	104045	702-702	0.166667	45.9
rpl13-rps9_p5	rpl13-rps9	5	CCTGGCCATCCTGGTAAATC	TTGGCCAGTTAATCCACCAC	20	20	55.0	50.0	58.0	58.1	5.5	5.1	0.095246	103683	104045	849-849	0.166667	45.9
clpC_p1	clpC	1	TAGCACGCAAAAGTATCGCT	AGCTAATGCAAGTCCTTCCG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.034421	108558	108677	1010-1010	0.166667	46.4
clpC_p2	clpC	2	GCAGTAAATGAAGCCCGAGA	AGCTAATGCAAGTCCTTCCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054014	108558	108677	855-855	0.166667	46.2
clpC_p3	clpC	3	TGAAGCCCGAGACATGAATG	AGCTAATGCAAGTCCTTCCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.058258	108558	108677	847-847	0.166667	46.2
clpC_p4	clpC	4	AGCAGTAAATGAAGCCCGAG	AGCTAATGCAAGTCCTTCCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.059677	108558	108677	856-856	0.166667	46.2
clpC_p5	clpC	5	GAAGCCCGAGACATGAATGT	AGCTAATGCAAGTCCTTCCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.059677	108558	108677	846-846	0.166667	46.2
psaC-psbA_p1	psaC-psbA	1	TGAGCCTGATACACGAAACG	TCGTAGCTGCACATGGTTAC	20	20	50.0	50.0	58.0	58.0	0.0	1.0	0.013783	117072	117872	1857-1857	0.166667	46.6
psaC-psbA_p2	psaC-psbA	2	TGAGCCTGATACACGAAACG	CGTAGCTGCACATGGTTACT	20	20	50.0	50.0	58.0	58.0	0.0	1.0	0.019346	117072	117872	1856-1856	0.166667	46.5
psaC-psbA_p3	psaC-psbA	3	TGAGCCTGATACACGAAACG	TGCATCTTACATGGGTCGTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.031965	117072	117872	2231-2231	0.166667	46.4
psaC-psbA_p4	psaC-psbA	4	TTGGGATGGATGTAAAGCCG	TCGTAGCTGCACATGGTTAC	20	20	50.0	50.0	57.9	58.0	0.0	1.0	0.055917	117072	117872	1299-1299	0.166667	46.2
psaC-psbA_p5	psaC-psbA	5	TTGGGATGGATGTAAAGCCG	CGTAGCTGCACATGGTTACT	20	20	50.0	50.0	57.9	58.0	0.0	1.0	0.061479	117072	117872	1298-1298	0.166667	46.2