Hotspot

rps19-ycf88

Genome · 92891-92903 · 13 bp

Back to Cyclostephanos

Pi 0.25

MarkerSeek score 80.10

Primer available yes

Label rps19-ycf88

Feature Metadata

label_name: rps19-ycf88
feature_type: igs
region: Genome
length_bp: 13
variable_sites: 3
pi: 0.25
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-ycf88_p1	TCACATATTAAAGCCGACAA	ATGTGGAAACTTTTTGGAAAA	35.0	28.6	52.6	52.6	95-96 bp	1.0	43.4
rps19-ycf88_p2	TCACATATTAAAGCCGACAA	TGTGGAAACTTTTTGGAAAAT	35.0	28.6	52.6	52.6	94-95 bp	1.0	43.4
rps19-ycf88_p3	TCACATATTAAAGCCGACAA	AATGTGGAAACTTTTTGGAAA	35.0	28.6	52.6	52.6	96-97 bp	1.0	43.3
rps19-ycf88_p4	ACATTTAAATCACATATTAAAGCCGAC	ATGTTAACTCTAATCGTGCCA	29.6	38.1	57.3	55.0	80 bp	0.5	20.0
rps19-ycf88_p5	ACATTTAAATCACATATTAAAGCCGA	ATGTTAACTCTAATCGTGCCA	26.9	38.1	56.1	55.0	80 bp	0.5	20.0