Hotspot

thiS-psb28

Genome · 85038-85536 · 499 bp

Back to Cyclostephanos

Pi 0.164

MarkerSeek score 79.30

Primer available yes

Label thiS-psb28

Feature Metadata

label_name: thiS-psb28
feature_type: igs
region: Genome
length_bp: 499
variable_sites: 80
pi: 0.164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
thiS-psb28_p1	TCACAACCCACTGCCTTAAC	CTTTGACTCCGCCATTCGTA	50.0	50.0	58.0	58.0	1299-1340 bp	1.0	88.2
thiS-psb28_p2	TCACAACCCACTGCCTTAAC	TTTGACTCCGCCATTCGTAG	50.0	50.0	58.0	58.0	1298-1339 bp	1.0	88.2
thiS-psb28_p3	TCACAACCCACTGCCTTAAC	CCTGCCACCCCAGTTTTTAT	50.0	50.0	58.0	58.1	1270-1311 bp	1.0	87.9
thiS-psb28_p4	GACCAGGCCTAGCAATCTTC	CTTTGACTCCGCCATTCGTA	55.0	50.0	58.0	58.0	1502 bp	0.5	59.6
thiS-psb28_p5	GACCAGGCCTAGCAATCTTC	TTTGACTCCGCCATTCGTAG	55.0	50.0	58.0	58.0	1501 bp	0.5	59.6