Hotspot

ycf46-ccs1

Genome · 78958-78964 · 7 bp

Back to Cyclostephanos

Pi 0.143

MarkerSeek score 79.20

Primer available yes

Label ycf46-ccs1

Feature Metadata

label_name: ycf46-ccs1
feature_type: igs
region: Genome
length_bp: 7
variable_sites: 1
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf46-ccs1_p1	AAAATTGGGCAGTCTCTGGG	ACTTAAAGCGGCAATCATCA	50.0	40.0	58.1	55.7	147 bp	1.0	61.8
ycf46-ccs1_p2	AAAATTGGGCAGTCTCTGGG	ACTTAAAGCGGCAATCATCAA	50.0	38.1	58.1	56.5	147 bp	1.0	60.0
ycf46-ccs1_p3	AAAATTGGGCAGTCTCTGGG	ACTTAAAGCGGCAATCATCAAT	50.0	36.4	58.1	57.0	147 bp	1.0	56.1
ycf46-ccs1_p4	GCGAATTCGTCTTGCTTCTT	ACTTAAAGCGGCAATCATCA	45.0	40.0	57.1	55.7	128 bp	1.0	55.4
ycf46-ccs1_p5	GGCGAATTCGTCTTGCTTCT	ACTTAAAGCGGCAATCATCA	50.0	40.0	58.9	55.7	129 bp	1.0	55.1