Hotspot

trnP-UGG-ycf89

Genome · 65154-65639 · 486 bp

Back to Cyclostephanos

Pi 0.105

MarkerSeek score 79.40

Primer available yes

Label trnP-UGG-ycf89

Feature Metadata

label_name: trnP-UGG-ycf89
feature_type: igs
region: Genome
length_bp: 486
variable_sites: 50
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-ycf89_p1	TTACGTGCGGTGAGTACAAG	ATACATCGCGGCACAATTCT	50.0	45.0	57.9	58.0	1438 bp	0.5	59.2
trnP-UGG-ycf89_p2	GTTGGATTGGATGGGTTGGT	ATACATCGCGGCACAATTCT	50.0	45.0	58.1	58.0	1467 bp	0.5	59.1
trnP-UGG-ycf89_p3	TGGTTGGATTGGATGGGTTG	ATACATCGCGGCACAATTCT	50.0	45.0	58.1	58.0	1469 bp	0.5	59.1
trnP-UGG-ycf89_p4	GCGGTGAGTACAAGTGCTAA	ATACATCGCGGCACAATTCT	50.0	45.0	57.9	58.0	1432 bp	0.5	59.0
trnP-UGG-ycf89_p5	TTACGTGCGGTGAGTACAAG	GATCACGTGCCTCGAAATCT	50.0	50.0	57.9	58.1	1159 bp	0.5	59.0