Hotspot

psaJ-trnP-UGG

Genome · 64964-65079 · 116 bp

Back to Cyclostephanos

Pi 0.112

MarkerSeek score 77.00

Primer available yes

Label psaJ-trnP-UGG

Feature Metadata

label_name: psaJ-trnP-UGG
feature_type: igs
region: Genome
length_bp: 116
variable_sites: 13
pi: 0.112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-trnP-UGG_p1	CGATTTTTCCCAGATATGTTAGGA	GGTAGAGTACCTGCTTTGGG	37.5	55.0	56.6	57.3	208-229 bp	1.0	44.8
psaJ-trnP-UGG_p2	CGATTTTTCCCAGATATGTTAGG	GGTAGAGTACCTGCTTTGGG	39.1	55.0	55.2	57.3	208-229 bp	1.0	44.8
psaJ-trnP-UGG_p3	TCGATTTTTCCCAGATATGTTAGGA	GTAGAGTACCTGCTTTGGGAG	36.0	52.4	57.9	57.5	208-229 bp	1.0	44.8
psaJ-trnP-UGG_p4	TCGATTTTTCCCAGATATGTTAGGA	GGTAGAGTACCTGCTTTGGG	36.0	55.0	57.9	57.3	209-230 bp	1.0	44.7
psaJ-trnP-UGG_p5	TCGATTTTTCCCAGATATGTTAGG	GGTAGAGTACCTGCTTTGGG	37.5	55.0	56.6	57.3	209-230 bp	1.0	44.7