Hotspot

secG-trnfM-CAU

Genome · 55590-55706 · 117 bp

Back to Cyclostephanos

Pi 0.145
MarkerSeek score 79.50
Primer available yes
Label secG-trnfM-CAU

Feature Metadata

label_name
secG-trnfM-CAU
feature_type
igs
region
Genome
length_bp
117
variable_sites
16
pi
0.145
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
secG-trnfM-CAU_p1 TATGCCCCCATCGTCTAGAG TACCAGACTGCTCTACTCCG 55.0 55.0 58.1 58.0 536-538 bp 1.0 87.6
secG-trnfM-CAU_p2 CAATCGCTACTGCAGATGGT TACCAGACTGCTCTACTCCG 50.0 55.0 58.1 58.0 811 bp 1.0 86.6
secG-trnfM-CAU_p3 CAATCGCTACTGCAGATGGT CATTGGCCTTCGGGTTATGA 50.0 50.0 58.1 57.9 842 bp 1.0 86.4
secG-trnfM-CAU_p4 CAATCGCTACTGCAGATGGT AATCGACCAACTCTGCCATC 50.0 50.0 58.1 58.0 1425 bp 1.0 84.1
secG-trnfM-CAU_p5 TATGCCCCCATCGTCTAGAG AATCGACCAACTCTGCCATC 55.0 50.0 58.1 58.0 1150-1152 bp 1.0 83.8