Hotspot

trnI-CAU-trnS-GCU

Genome · 49770-49794 · 25 bp

Back to Cyclostephanos

Pi 0.16
MarkerSeek score 78.60
Primer available yes
Label trnI-CAU-trnS-GCU

Feature Metadata

label_name
trnI-CAU-trnS-GCU
feature_type
igs
region
Genome
length_bp
25
variable_sites
4
pi
0.16
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-trnS-GCU_p1 GACTCATAAGCCCAAGGTCG CTTTCGACCACTCAGCCATT 55.0 50.0 58.1 58.2 95 bp 1.0 81.4
trnI-CAU-trnS-GCU_p2 GACTCATAAGCCCAAGGTCG TTCGACCACTCAGCCATTTC 55.0 50.0 58.1 58.2 93 bp 1.0 81.4
trnI-CAU-trnS-GCU_p3 ATAAGCCCAAGGTCGTAGGT CTTTCGACCACTCAGCCATT 50.0 50.0 58.1 58.2 90 bp 1.0 81.1
trnI-CAU-trnS-GCU_p4 ATAAGCCCAAGGTCGTAGGT TTCGACCACTCAGCCATTTC 50.0 50.0 58.1 58.2 88 bp 1.0 81.1
trnI-CAU-trnS-GCU_p5 GACTCATAAGCCCAAGGTCG GACCACTCAGCCATTTCTCC 55.0 55.0 58.1 58.3 90 bp 1.0 81.0