Hotspot

pbf1-psbH

Genome · 19132-19215 · 84 bp

Back to Cyclostephanos

Pi 0.0952

MarkerSeek score 77.70

Primer available yes

Label pbf1-psbH

Feature Metadata

label_name: pbf1-psbH
feature_type: igs
region: Genome
length_bp: 84
variable_sites: 8
pi: 0.0952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pbf1-psbH_p1	AAGCAGGTCCAAAAGCAGTA	TCAGCATTTAACGGGCGTAA	45.0	45.0	57.3	57.9	225 bp	1.0	76.8
pbf1-psbH_p2	AAGCAGGTCCAAAAGCAGTA	TTCAGCATTTAACGGGCGTA	45.0	45.0	57.3	57.9	226 bp	1.0	76.8
pbf1-psbH_p3	GAAGCAGGTCCAAAAGCAGT	TCAGCATTTAACGGGCGTAA	50.0	45.0	58.7	57.9	226 bp	1.0	76.6
pbf1-psbH_p4	GAAGCAGGTCCAAAAGCAGT	TTCAGCATTTAACGGGCGTA	50.0	45.0	58.7	57.9	227 bp	1.0	76.6
pbf1-psbH_p5	AGCAGGTCCAAAAGCAGTAT	TCAGCATTTAACGGGCGTAA	45.0	45.0	57.1	57.9	224 bp	1.0	75.0