Hotspot

ssrA-trnR-CCG

Genome · 6233-6286 · 54 bp

Back to Cyclostephanos

Pi 0.113

MarkerSeek score 77.00

Primer available yes

Label ssrA-trnR-CCG

Feature Metadata

label_name: ssrA-trnR-CCG
feature_type: igs
region: Genome
length_bp: 54
variable_sites: 6
pi: 0.113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ssrA-trnR-CCG_p1	GTCGAAACCAAATCAGTCCC	GGTTCGATTCCCTTCATGCT	50.0	50.0	56.7	58.0	95-96 bp	1.0	75.3
ssrA-trnR-CCG_p2	TGTCGAAACCAAATCAGTCCC	GGTTCGATTCCCTTCATGCT	47.6	50.0	58.5	58.0	96-97 bp	1.0	73.5
ssrA-trnR-CCG_p3	GTCGAAACCAAATCAGTCCC	GGGGTTCGATTCCCTTCATG	50.0	55.0	56.7	58.3	97-98 bp	1.0	73.0
ssrA-trnR-CCG_p4	GTCGAAACCAAATCAGTCCC	ATTCTGAAAGTCGGGGGTTC	50.0	50.0	56.7	57.5	110-111 bp	1.0	70.9
ssrA-trnR-CCG_p5	GTCGAAACCAAATCAGTCCC	GATTCTGAAAGTCGGGGGTT	50.0	50.0	56.7	57.5	111-112 bp	1.0	70.9