Hotspot

ndhF

SSC · 111258-113504 · 2247 bp

Back to Cyclorhiza

Pi 0.0111

MarkerSeek score 51.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 43
pi: 0.0111
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCCTGCCCACACTAGAAAAA	GGGGGTAGGATCCGGTAAAT	50.0	55.0	57.7	58.3	2930-2976 bp	1.0	77.4
ndhF_p2	TCCCTGCCCACACTAGAAAA	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.6	58.3	2931-2977 bp	1.0	75.2
ndhF_p3	TACGTATCCGCCTAGCTTGT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.3	58.3	2983 bp	0.25	45.4
ndhF_p4	CCGCCTAGCTTGTCAACTTT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.5	58.3	2976 bp	0.25	44.1
ndhF_p5	TATCCGCCTAGCTTGTCAAC	GGGGGTAGGATCCGGTAAAT	50.0	55.0	57.4	58.3	2979 bp	0.25	43.1