Hotspot

trnR-ACG-5S ribosomal RNA

IRa · 131976-132246 · 271 bp

Back to Cyclorhiza

Pi 0.0387
MarkerSeek score 79.00
Primer available yes
Label trnR-ACG-5S ribosomal RNA

Feature Metadata

label_name
trnR-ACG-5S ribosomal RNA
feature_type
igs
region
IRa
length_bp
271
variable_sites
21
pi
0.0387
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-5S ribosomal RNA_p1 CCACGTGCTCTAATCCTCTG GGTGACGATACTGTAGGGGA 55.0 55.0 57.8 57.9 342-355 bp 1.0 86.6
trnR-ACG-5S ribosomal RNA_p2 CCACGTGCTCTAATCCTCTG AATCCATCCCGAACTTGGTG 55.0 50.0 57.8 57.9 377-390 bp 1.0 85.6
trnR-ACG-5S ribosomal RNA_p3 CCACGTGCTCTAATCCTCTG ACCAATCCATCCCGAACTTG 55.0 50.0 57.8 57.9 380-393 bp 1.0 85.6
trnR-ACG-5S ribosomal RNA_p4 CCACGTGCTCTAATCCTCTG CAATCCATCCCGAACTTGGT 55.0 50.0 57.8 57.9 378-391 bp 1.0 85.6
trnR-ACG-5S ribosomal RNA_p5 CCACGTGCTCTAATCCTCTG CACCAATCCATCCCGAACTT 55.0 50.0 57.8 57.9 381-394 bp 1.0 85.6