Hotspot

ndhG-ndhI

SSC · 120298-120709 · 412 bp

Back to Cyclorhiza

Pi 0.0214

MarkerSeek score 61.30

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 412
variable_sites: 15
pi: 0.0214
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GCAATCGCACCTATTAACGC	TTCTTTGGGGCGTTTACCAA	50.0	45.0	58.0	57.9	983-1016 bp	1.0	83.8
ndhG-ndhI_p2	GCAATCGCACCTATTAACGC	TTTCTTTGGGGCGTTTACCA	50.0	45.0	58.0	57.9	984-1017 bp	1.0	83.8
ndhG-ndhI_p3	CATTTATGGCCCCCACGTAA	ACAAACAGTACGAGCTGCAA	50.0	45.0	57.9	58.1	1063-1096 bp	1.0	83.2
ndhG-ndhI_p4	GCAATCGCACCTATTAACGC	AACAAACAGTACGAGCTGCA	50.0	45.0	58.0	58.1	1365-1398 bp	1.0	83.0
ndhG-ndhI_p5	GCAATCGCACCTATTAACGC	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.0	58.1	1364-1397 bp	1.0	83.0