Hotspot

ndhF-rpl32

SSC · 113505-114684 · 1180 bp

Back to Cyclorhiza

Pi 0.0193

MarkerSeek score 63.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1180
variable_sites: 41
pi: 0.0193
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAAGTCCCGCTCCTATTAACA	AGGCTGCCCAATATCCTTTC	47.6	50.0	57.2	57.6	1342-1386 bp	1.0	65.2
ndhF-rpl32_p2	AAGTCCCGCTCCTATTAACA	AGGCTGCCCAATATCCTTTC	45.0	50.0	55.9	57.6	1341-1385 bp	1.0	63.3
ndhF-rpl32_p3	AGAAGTCCCGCTCCTATTAAC	AGGCTGCCCAATATCCTTTC	47.6	50.0	56.9	57.6	1343-1387 bp	1.0	62.9
ndhF-rpl32_p4	GAAGTCCCGCTCCTATTAACA	GCTGCCCAATATCCTTTCCC	47.6	55.0	57.2	58.7	1340-1384 bp	1.0	62.9
ndhF-rpl32_p5	GAAGTCCCGCTCCTATTAACA	GGCTGCCCAATATCCTTTCC	47.6	55.0	57.2	58.7	1341-1385 bp	1.0	62.9