Hotspot

5S ribosomal RNA-trnR-ACG

IRb · 108084-108354 · 271 bp

Back to Cyclorhiza

Pi 0.0387
MarkerSeek score 79.00
Primer available yes
Label 5S ribosomal RNA-trnR-ACG

Feature Metadata

label_name
5S ribosomal RNA-trnR-ACG
feature_type
igs
region
IRb
length_bp
271
variable_sites
21
pi
0.0387
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
5S ribosomal RNA-trnR-ACG_p1 GGTGACGATACTGTAGGGGA CCACGTGCTCTAATCCTCTG 55.0 55.0 57.9 57.8 342-355 bp 1.0 86.6
5S ribosomal RNA-trnR-ACG_p2 AATCCATCCCGAACTTGGTG CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 377-390 bp 1.0 85.6
5S ribosomal RNA-trnR-ACG_p3 ACCAATCCATCCCGAACTTG CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 380-393 bp 1.0 85.6
5S ribosomal RNA-trnR-ACG_p4 CAATCCATCCCGAACTTGGT CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 378-391 bp 1.0 85.6
5S ribosomal RNA-trnR-ACG_p5 CACCAATCCATCCCGAACTT CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 381-394 bp 1.0 85.6