Hotspot

rpl22-rps19

LSC · 84871-84938 · 68 bp

Back to Cyclorhiza

Pi 0.0224

MarkerSeek score 64.00

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 68
variable_sites: 3
pi: 0.0224
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TGACCTAAAGCGTATACTTCTGT	AAATTTCCGAGGACACGCAA	39.1	45.0	57.1	58.4	155-167 bp	1.0	51.0
rpl22-rps19_p2	TGACCTAAAGCGTATACTTCTGT	AATTTCCGAGGACACGCAAA	39.1	45.0	57.1	58.4	154-166 bp	1.0	51.0
rpl22-rps19_p3	TGACCTAAAGCGTATACTTCTGT	ATTTCCGAGGACACGCAAAA	39.1	45.0	57.1	58.4	153-165 bp	1.0	51.0
rpl22-rps19_p4	TGACCTAAAGCGTATACTTCTGT	TAAATTTCCGAGGACACGCA	39.1	45.0	57.1	57.5	156-168 bp	1.0	50.5
rpl22-rps19_p5	TGACCTAAAGCGTATACTTCTGT	TTTCCGAGGACACGCAAAAA	39.1	45.0	57.1	58.6	152-164 bp	1.0	49.3