Hotspot

clpP-psbB

LSC · 72937-73408 · 472 bp

Back to Cyclorhiza

Pi 0.0106

MarkerSeek score 67.20

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 472
variable_sites: 9
pi: 0.0106
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	GCACTATACGTCAACCCAGG	CGATACCAAGGCAAACCCAT	55.0	50.0	58.1	58.2	550-567 bp	1.0	81.3
clpP-psbB_p2	CAGGACTTCGAAAAGGCACT	CGATACCAAGGCAAACCCAT	50.0	50.0	58.1	58.2	515-532 bp	1.0	81.1
clpP-psbB_p3	AGGCACTTTTGGAACACCAA	CGATACCAAGGCAAACCCAT	45.0	50.0	58.1	58.2	502-519 bp	1.0	81.0
clpP-psbB_p4	GTCAACCCAGGATGCATCTT	CGATACCAAGGCAAACCCAT	50.0	50.0	57.9	58.2	541-558 bp	1.0	80.8
clpP-psbB_p5	AGGATGCATCTTCCTCTCCA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	533-550 bp	1.0	80.4