Hotspot

ndhC-trnV-UAC

LSC · 51987-53163 · 1177 bp

Back to Cyclorhiza

Pi 0.0149
MarkerSeek score 58.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1177
variable_sites
31
pi
0.0149
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 31.8 55.0 53.9 58.1 1222-1244 bp 1.0 42.9
ndhC-trnV-UAC_p2 TGCCAAAATAGGAATAAGACTTGA CCGAGAAAGTCTACGGTTCG 33.3 55.0 56.3 58.1 1271-1293 bp 1.0 42.8
ndhC-trnV-UAC_p3 TGCCAAAATAGGAATAAGACTTG CCGAGAAAGTCTACGGTTCG 34.8 55.0 54.9 58.1 1271-1293 bp 1.0 42.8
ndhC-trnV-UAC_p4 ATGCCAAAATAGGAATAAGACTTGA CCGAGAAAGTCTACGGTTCG 32.0 55.0 56.8 58.1 1272-1294 bp 1.0 42.8
ndhC-trnV-UAC_p5 TGCCAAAATAGGAATAAGACTTGAT CCGAGAAAGTCTACGGTTCG 32.0 55.0 56.8 58.1 1271-1293 bp 1.0 42.8