Hotspot

trnF-GAA-ndhJ

LSC · 49938-50309 · 372 bp

Back to Cyclorhiza

Pi 0.0157

MarkerSeek score 63.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 372
variable_sites: 10
pi: 0.0157
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGGATAGGATGGCCTTTACG	55.0	50.0	58.0	56.8	511-532 bp	1.0	72.2
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	GGATAGGATGGCCTTTACGT	55.0	50.0	58.0	56.8	510-531 bp	1.0	72.2
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	475-496 bp	1.0	72.0
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	474-495 bp	1.0	71.9
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGATAGGATGGCC	55.0	47.6	58.0	57.7	518-539 bp	1.0	71.6