Hotspot

trnE-UUC-trnT-GGU

LSC · 31772-32598 · 827 bp

Back to Cyclorhiza

Pi 0.0133

MarkerSeek score 63.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 827
variable_sites: 18
pi: 0.0133
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	931-957 bp	1.0	82.9
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	896-922 bp	1.0	82.3
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	928-954 bp	1.0	81.8
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	TTGAACCGATGACTTACGCC	50.0	50.0	57.8	58.3	933-959 bp	1.0	80.7
trnE-UUC-trnT-GGU_p5	TTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	57.4	58.0	966-992 bp	1.0	79.7