Hotspot

atpF-atpH

LSC · 13543-13918 · 376 bp

Back to Cyclorhiza

Pi 0.0116

MarkerSeek score 63.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 376
variable_sites: 8
pi: 0.0116
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGCCCAAAGAAACGAAAGA	GCCTGGTTGTAGCATTAGCA	45.0	50.0	57.9	58.2	457-469 bp	1.0	79.6
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	488-500 bp	1.0	79.0
atpF-atpH_p3	GTGGCCCAAAGAAACGAAAG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	458-470 bp	1.0	79.0
atpF-atpH_p4	TGGCCCAAAGAAACGAAAGA	TGGCCTGGTTGTAGCATTAG	45.0	50.0	57.9	57.6	459-471 bp	1.0	78.2
atpF-atpH_p5	AGTGGCCCAAAGAAACGAAA	GCCTGGTTGTAGCATTAGCA	45.0	50.0	58.2	58.2	459-471 bp	1.0	78.2