Hotspot

psbK-psbI

LSC · 8180-8593 · 414 bp

Back to Cyclorhiza

Pi 0.027

MarkerSeek score 64.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 21
pi: 0.027
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGAAAAGTTTGAGAGTAAGCA	50.0	36.4	57.9	56.2	467-481 bp	1.0	53.4
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	ACGAAAAGTTTGAGAGTAAGCA	45.0	36.4	58.3	56.2	464-478 bp	1.0	51.4
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	ACGAAAAGTTTGAGAGTAAGCA	45.0	36.4	58.3	56.2	465-479 bp	1.0	51.4
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	ACGAAAAGTTTGAGAGTAAGCA	45.0	36.4	58.3	56.2	466-480 bp	1.0	51.4
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	AACGAAAAGTTTGAGAGTAAGCA	50.0	34.8	57.9	56.9	468-482 bp	1.0	50.9