Hotspot

trnH-GUG-psbA

LSC · 79-452 · 374 bp

Back to Cyclorhiza

Pi 0.0401

MarkerSeek score 72.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 374
variable_sites: 25
pi: 0.0401
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCCTCTAGATCTAGCTGCTGT	55.0	52.4	58.1	58.1	437-444 bp	1.0	76.3
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCCTCTAGATCTAGCTGCTGT	50.0	52.4	57.9	58.1	451-458 bp	1.0	76.1
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCCTCTAGATCTAGCTGCTGT	50.0	52.4	57.9	58.1	450-457 bp	1.0	76.1
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCCTCTAGATCTAGCTGCTGT	50.0	52.4	57.9	58.1	452-459 bp	1.0	76.1
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TCCCTCTAGATCTAGCTGCTG	55.0	52.4	58.1	57.8	438-445 bp	1.0	76.1