Hotspot

ycf1

SSC · 123608-129220 · 5613 bp

Back to Cyclopogon

Pi 0.0223

MarkerSeek score 52.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5613
variable_sites: 124
pi: 0.0223
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTTCGGGGAGAACCAAAGG	ACGTTACTCCCAACAATCGG	50.0	50.0	58.0	57.9	766-772 bp	1.0	81.5
ycf1_p2	AGGTTGTTTTCGGGGAGAAC	ACGTTACTCCCAACAATCGG	50.0	50.0	58.0	57.9	772-778 bp	1.0	81.4
ycf1_p3	TTTTCGGGGAGAACCAAAGG	CAATCGGATTTTCGTCGCAG	50.0	50.0	58.0	57.9	753 bp	0.5	59.3
ycf1_p4	AGGTTGTTTTCGGGGAGAAC	CAATCGGATTTTCGTCGCAG	50.0	50.0	58.0	57.9	759 bp	0.5	59.2
ycf1_p5	TTTTCGGGGAGAACCAAAGG	CGGATTTTCGTCGCAGTCTA	50.0	50.0	58.0	58.1	749 bp	0.5	59.2