Hotspot

rpl32-trnL-UAG

SSC · 113292-114065 · 774 bp

Back to Cyclopogon

Pi 0.033
MarkerSeek score 73.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
774
variable_sites
25
pi
0.033
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGCTTTTATTGCCGATAAAGATCC TCCTAAGAGCAGCGTGTCTA 37.5 50.0 57.7 57.9 906-920 bp 1.0 48.0
rpl32-trnL-UAG_p2 GCTTTTATTGCCGATAAAGATCCT TCCTAAGAGCAGCGTGTCTA 37.5 50.0 57.7 57.9 905-919 bp 1.0 48.0
rpl32-trnL-UAG_p3 AGAAAAAGCTTTTATTGCCGA TCCTAAGAGCAGCGTGTCTA 33.3 50.0 54.7 57.9 912-926 bp 1.0 47.8
rpl32-trnL-UAG_p4 AGCTTTTATTGCCGATAAAGATCC AGCAGCGTGTCTACCAATTT 37.5 45.0 57.7 57.8 899-913 bp 1.0 47.6
rpl32-trnL-UAG_p5 GCTTTTATTGCCGATAAAGATCCT AGCAGCGTGTCTACCAATTT 37.5 45.0 57.7 57.8 898-912 bp 1.0 47.6