Hotspot

rps11-rpl36

LSC · 79302-79468 · 167 bp

Back to Cyclopogon

Pi 0.0838

MarkerSeek score 73.70

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 14
pi: 0.0838
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTGTATTCTTGCGTAAACCA	GACATAAACAGAGACAGGGGT	42.9	47.6	56.9	57.0	234 bp	1.0	52.4
rps11-rpl36_p2	ACGTGTATTCTTGCGTAAACC	GACATAAACAGAGACAGGGGT	42.9	47.6	56.9	57.0	235 bp	1.0	52.3
rps11-rpl36_p3	CCAATACGTGTATTCTTGCGT	GACATAAACAGAGACAGGGGT	42.9	47.6	56.7	57.0	240 bp	1.0	50.6
rps11-rpl36_p4	ACCAATACGTGTATTCTTGCG	GACATAAACAGAGACAGGGGT	42.9	47.6	56.7	57.0	241 bp	1.0	50.5
rps11-rpl36_p5	CGTGTATTCTTGCGTAAACCA	ACATAAACAGAGACAGGGGT	42.9	45.0	56.9	55.7	233 bp	1.0	50.3