Hotspot

rpl20-rps12

LSC · 68181-68978 · 798 bp

Back to Cyclopogon

Pi 0.0251

MarkerSeek score 59.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 20
pi: 0.0251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GAGTGATCCACAAACGACGA	TACAAAAGATGCCCGTACGG	50.0	50.0	57.9	58.0	1591-1601 bp	1.0	80.8
rpl20-rps12_p2	GAGTGATCCACAAACGACGA	ATCGATCTAAACCAACCCGC	50.0	50.0	57.9	58.1	1158-1165 bp	1.0	80.7
rpl20-rps12_p3	GAGTGATCCACAAACGACGA	CAAATTCCATTGCAGAGCCG	50.0	50.0	57.9	58.1	1620-1630 bp	1.0	80.2
rpl20-rps12_p4	TCCTCGCTTAACTCTGGTCA	TACAAAAGATGCCCGTACGG	50.0	50.0	58.1	58.0	1422 bp	0.5	59.3
rpl20-rps12_p5	TCCTCGCTTAACTCTGGTCA	ATCGATCTAAACCAACCCGC	50.0	50.0	58.1	58.1	989 bp	0.5	58.9