Hotspot

accD-psaI

LSC · 58202-58861 · 660 bp

Back to Cyclopogon

Pi 0.0413

MarkerSeek score 79.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 26
pi: 0.0413
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTTGACCCAATCGTACCACG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	796-826 bp	1.0	83.1
accD-psaI_p2	TTTGACCCAATCGTACCACG	TGCAATTGCCGGAAAGACTA	50.0	45.0	58.2	57.8	786-816 bp	1.0	82.4
accD-psaI_p3	TGACCCAATCGTACCACGTA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.5	57.9	794-824 bp	1.0	81.1
accD-psaI_p4	TTTGACCCAATCGTACCACG	CATTGCAATTGCCGGAAAGA	50.0	45.0	58.2	57.6	789-819 bp	1.0	81.0
accD-psaI_p5	TTTGACCCAATCGTACCACG	ATTGCAATTGCCGGAAAGAC	50.0	45.0	58.2	57.6	788-818 bp	1.0	81.0