Hotspot

psaA-ycf3

LSC · 40680-41330 · 651 bp

Back to Cyclopogon

Pi 0.0262

MarkerSeek score 72.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 651
variable_sites: 17
pi: 0.0262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	ACAGGCTTGGTTCGATCAAG	50.0	50.0	57.9	58.2	842 bp	1.0	79.5
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	ATAGCGCTTACTCCAGGGAA	50.0	50.0	57.9	58.2	799 bp	1.0	79.4
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCAGGGAAT	50.0	50.0	57.9	58.2	798 bp	1.0	79.4
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	GTTCGATCAAGCTGCTGAGT	50.0	50.0	57.9	58.3	833 bp	1.0	78.7
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	802 bp	1.0	78.2