Hotspot

rpoB-trnC-GCA

LSC · 26258-27371 · 1114 bp

Back to Cyclopogon

Pi 0.0257

MarkerSeek score 60.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1114
variable_sites: 28
pi: 0.0257
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCAAATTAAGACCGCGGGTA	ACAGACCTAGTTCCTTGCCT	50.0	50.0	58.0	58.0	1556-1567 bp	1.0	82.7
rpoB-trnC-GCA_p2	CCAAATTAAGACCGCGGGTA	TACTCAGAGCCTCTCGTGAG	50.0	55.0	58.0	58.0	2000-2043 bp	1.0	82.5
rpoB-trnC-GCA_p3	CCAAATTAAGACCGCGGGTA	TGAGTACTCAGAGCCTCTCG	50.0	55.0	58.0	58.0	2004-2047 bp	1.0	82.5
rpoB-trnC-GCA_p4	CCAAATTAAGACCGCGGGTA	GAGTACTCAGAGCCTCTCGT	50.0	55.0	58.0	58.0	2003-2046 bp	1.0	82.5
rpoB-trnC-GCA_p5	CCCCTACACAGGCAAATTGT	TACTCAGAGCCTCTCGTGAG	50.0	55.0	58.1	58.0	2439-2482 bp	1.0	81.6