Hotspot

atpH-atpI

LSC · 13375-13923 · 549 bp

Back to Cyclopogon

Pi 0.0358

MarkerSeek score 67.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 549
variable_sites: 19
pi: 0.0358
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCAGCAGCAATAACGGAAG	ATGGAGGGTCATCATTGA	50.0	44.4	58.3	52.2	604-609 bp	1.0	44.9
atpH-atpI_p2	AATCCAGCAGCAATAACGGA	ATGGAGGGTCATCATTGA	45.0	44.4	57.6	52.2	606-611 bp	1.0	44.9
atpH-atpI_p3	ATCCAGCAGCAATAACGGAA	ATGGAGGGTCATCATTGA	45.0	44.4	57.6	52.2	605-610 bp	1.0	44.9
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	ATGGAGGGTCATCATTGA	45.0	44.4	57.9	52.2	610-615 bp	1.0	44.8
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	ATGGAGGGTCATCATTGA	50.0	44.4	57.8	52.2	609-614 bp	1.0	44.8