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psbK-psbI

LSC · 7320-7845 · 526 bp

Back to Cyclopogon

Pi 0.0296

MarkerSeek score 72.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 526
variable_sites: 14
pi: 0.0296
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGCCCGGGATCATTAGATA	50.0	50.0	57.9	58.1	594-646 bp	1.0	84.8
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TACGCCCGGGATCATTAGAT	50.0	50.0	57.9	58.1	595-647 bp	1.0	84.8
psbK-psbI_p3	GCCAAATTACCCGAGGCTTA	ACGCCCGGGATCATTAGATA	50.0	50.0	58.0	58.1	681-733 bp	1.0	84.7
psbK-psbI_p4	GCCAAATTACCCGAGGCTTA	TACGCCCGGGATCATTAGAT	50.0	50.0	58.0	58.1	682-734 bp	1.0	84.7
psbK-psbI_p5	GCCAAATTACCCGAGGCTTA	TTACGCCCGGGATCATTAGA	50.0	50.0	58.0	58.3	683-735 bp	1.0	82.9