Hotspot

trnK-UUU-rps16

LSC · 4309-4919 · 611 bp

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Pi 0.0287
MarkerSeek score 76.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
611
variable_sites
17
pi
0.0287
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAGGGCGCTCAACCTAC 50.0 52.6 58.2 58.1 752-804 bp 1.0 73.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAGGGCGCTCAACCTAC 50.0 52.6 58.2 58.1 751-803 bp 1.0 73.9
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GAAAAGGGCGCTCAACCTAC 50.0 55.0 58.2 59.2 753-805 bp 1.0 72.8
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GAAAAGGGCGCTCAACCTAC 50.0 55.0 58.2 59.2 752-804 bp 1.0 72.8
trnK-UUU-rps16_p5 AGGATCAGTCGCGGTCTTAT AAAAGGGCGCTCAACCTAC 50.0 52.6 58.3 58.1 837-889 bp 1.0 72.4