Hotspot

ycf1

IRa · 134468-141343 · 6876 bp

Back to Cyclocodon

Pi 0.0107

MarkerSeek score 44.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRa
length_bp: 6876
variable_sites: 73
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTCCGAGCCCATAGTCTAC	TCTTTCACAGAGTCCCTCGT	55.0	50.0	58.0	58.0	1129-1210 bp	1.0	81.8
ycf1_p2	ACCACTCGGTCTTTTGCAAT	TCTTTCACAGAGTCCCTCGT	45.0	50.0	58.0	58.0	1242-1323 bp	1.0	81.7
ycf1_p3	AGTCCCCAGAAAGCCCTTAT	TCTTTCACAGAGTCCCTCGT	50.0	50.0	58.0	58.0	1453-1534 bp	1.0	81.6
ycf1_p4	ATAGTCCCCAGAAAGCCCTT	TCTTTCACAGAGTCCCTCGT	50.0	50.0	58.0	58.0	1455-1536 bp	1.0	81.6
ycf1_p5	GTTTATGGGTTTGACGTGCG	TCTTTCACAGAGTCCCTCGT	50.0	50.0	58.0	58.0	1040 bp	0.5	59.6