Hotspot

rps3

LSC · 82030-82686 · 657 bp

Back to Cyclocodon

Pi 0.0259

MarkerSeek score 58.60

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 17
pi: 0.0259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	ATAGAAGGGAAGGGCAAGGT	CCACAAGATGACTATGCATTCG	50.0	45.5	58.0	57.5	739 bp	1.0	62.1
rps3_p2	TCGAATAGAAGGGAAGGGCA	CCACAAGATGACTATGCATTCG	50.0	45.5	58.1	57.5	743 bp	1.0	61.2
rps3_p3	TAGAAGGGAAGGGCAAGGTT	CCACAAGATGACTATGCATTCG	50.0	45.5	58.2	57.5	738 bp	1.0	60.3
rps3_p4	GAAGGGAAGGGCAAGGTTTA	CCACAAGATGACTATGCATTCG	50.0	45.5	57.4	57.5	736 bp	1.0	57.5
rps3_p5	ATAGAAGGGAAGGGCAAGGT	CACAAGATGACTATGCATTCGT	50.0	40.9	58.0	56.9	738 bp	1.0	56.8