Hotspot

trnL-UAG-rpl32

SSC · 124072-124920 · 849 bp

Back to Cyclocodon

Pi 0.0271

MarkerSeek score 66.70

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 849
variable_sites: 23
pi: 0.0271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	TCTAGGTCGGCGTTAAAAGC	50.0	50.0	58.0	58.0	968 bp	1.0	81.8
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	TCTAGGTCGGCGTTAAAAGC	50.0	50.0	58.0	58.0	969 bp	1.0	81.8
trnL-UAG-rpl32_p3	AGCGTGTCTACCGATTTCAC	CTAGGTCGGCGTTAAAAGCT	50.0	50.0	58.0	58.0	967 bp	1.0	81.8
trnL-UAG-rpl32_p4	CAGCGTGTCTACCGATTTCA	CTAGGTCGGCGTTAAAAGCT	50.0	50.0	58.0	58.0	968 bp	1.0	81.8
trnL-UAG-rpl32_p5	AGCGTGTCTACCGATTTCAC	GGCGTTAAAAGCTTTGGCAT	50.0	45.0	58.0	57.9	960 bp	1.0	81.2